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News 244 Publications 8 Members

Adenosine monophosphate forms ordered arrays in multilamellar lipid matrices: insights into assembly of nucleic acid for... PDF available through Get Fulltext Research

Toppozini, L Dies, H David Deamer Rheinstädter, Mc

Published in PLoS ONE

A fundamental question of biology is how nucleic acids first assembled and then were incorporated into the earliest forms of cellular life 4 billion years ago. The polymerization of nucleotides is a condensation reaction in which phosphodiester bonds are formed. This reaction cannot occur in aqueous solutions, but guided polymerization in an anhydr...

Fisher s theorems for multivariable, time- and space-dependent systems, with applications in population genetics and che...

Vlad, Mo Szedlacsek, Se Nader Pourmand Cavalli-Sforza, Ll Oefner, P Ross, J

Published in Proceedings of the National Academy of Sciences

We study different physical, chemical, or biological processes involving replication, transformation, and disappearance processes, as well as transport processes, and assume that the time and space dependence of the species densities are known. We derive two types of Fisher equations. The first type relates the average value of the time derivative ...

Characterization of synthetic DNA bar codes in Saccharomyces cerevisiae gene-deletion strains.

Eason, Rg Nader Pourmand Tongprasit, W Herman, Zs Anthony, K Jejelowo, O Davis, Rw Stolc, V

Published in Proceedings of the National Academy of Sciences

Incorporation of strain-specific synthetic DNA tags into yeast Saccharomyces cerevisiae gene-deletion strains has enabled identification of gene functions by massively parallel growth rate analysis. However, it is important to confirm the sequences of these tags, because mutations introduced during construction could lead to significant errors in h...

Liposomes from ionic, single-chain amphiphiles.

Hargreaves, Wr David Deamer

Published in Biochemistry

In studies of the minimum physiochemical requirements for lipid membrane formation, we have made liposomes from dilute, aqueous dispersions of C(8)-C(18) single-chain amphiphiles. In general, membrane formation from ionic soaps and detergents requires the presence of uncharged amphiphiles. Vesicles were characterized by phase-contrast microscopy, b...

Redox chemistry at liquid/liquid interfaces.

Volkov, Ag David Deamer

Published in Progress in Colloid & Polymer Science

The interface between two immiscible liquids with immobilized photosynthetic pigments can serve as the simplest model of a biological membrane convenient for the investigation of photoprocesses accompanied by spatial separation of charges. As it follows from thermodynamics, if the resolvation energies of substrates and products are very different, ...

Imaging defect formation in the template growth of NiSi2/Si(111): An application of quantum size microscopy.

Joel Kubby Wang, Yr Greene, Wj

Published in Physical Review B

Endogenously produced CO2 induces stationary phase in Tetrahymena cultures.

Gillies, Rj David Deamer

Published in Journal of Cellular Physiology

Media concentration of total soluble CO2 increases with culture age of Tetrahymena pyriformis. CO2 is a weak acid and is capable of acidifying intracellular pH (pHi). Changes in pHi have been demonstrated to affect cell metabolism and growth in many systems. For these reasons, we investigated whether the concentrations of CO2 produced in vitro were...

Ro 52kD autoantibodies are detected in a subset of ANA-negative sera.

Nader Pourmand Blomberg, S Rönnblom, L Karlsson-Parra, A Pettersson, I Wahren-Herlenius, M

Published in Scandinavian Journal of Rheumatology

To define Ro 52kD, Ro 60kD, and La specificities of autoantibodies within ANA-negative sera, samples from 64 ANA-negative but SSA positive patients undergoing investigation due to suspected CTD were analysed, using recombinant antigens and synthetic peptides by immunoblotting and ELISA. The sera were selected from 4025 sera submitted for routine AN...

Particle in a variable-size box: The influence of the tip in thin-film electron interferometry.

Joel Kubby Greene, Wj

Published in Physical Review B

Direct oligonucleotide synthesis onto super-paramagnetic beads.

Jensen, Ma Akhras, Ms Fukushima, M Nader Pourmand Davis, Rw

Published in Journal of Biotechnology

Super-paramagnetic beads (SPMB)s used for a variety of molecular diagnostic assays are prepared by attaching pre-synthesized oligonucleotides to the surface via a cumbersome and low efficient method of carbodiimide-mediated amide bond formation. To mainstream the process, we describe a novel procedure of direct oligonucleotide synthesis onto the su...

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