Gharizadeh, B Akhras, M Nourizad, N Ghaderi, M Yasuda, K Nyrén, P Nader Pourmand
Published in
Journal of Biotechnology
Pyrosequencing technology is a rather novel DNA sequencing method based on the sequencing-by-synthesis principle. This bioluminometric, real-time DNA sequencing technique employs a cascade of four enzymatic reactions producing sequence peak signals. The method has been proven highly suitable for single nucleotide polymorphism analysis and sequencin...
Jensen, Ma Akhras, Ms Fukushima, M Nader Pourmand Davis, Rw
Published in
Journal of Biotechnology
Super-paramagnetic beads (SPMB)s used for a variety of molecular diagnostic assays are prepared by attaching pre-synthesized oligonucleotides to the surface via a cumbersome and low efficient method of carbodiimide-mediated amide bond formation. To mainstream the process, we describe a novel procedure of direct oligonucleotide synthesis onto the su...