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Optimized Protocol for Simple Extraction of High-Quality Genomic DNA from Clostridium difficile for Whole-Genome Sequenc... PDF available through Get Fulltext Research

Sim, James Heng Chiak Anikst, Victoria Lohith, Akshar Pourmand, Nader Banaei, Niaz

Published in Journal of clinical microbiology

Successful sequencing of the Clostridium difficile genome requires high-quality genomic DNA (gDNA) as the starting material. gDNA extraction using conventional methods is laborious. We describe here an optimized method for the simple extraction of C. difficile gDNA using the QIAamp DNA minikit, which yielded high-quality sequence reads on the Illum...

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