Marcelin, Glendie DuBois, Rebecca Rubrum, Adam Russell, Charles J McElhaney, Janet E Webby, Richard J
Published in
PloS one
Exposure to contemporary seasonal influenza A viruses affords partial immunity to pandemic H1N1 2009 influenza A virus (pH1N1) infection. The impact of antibodies to the neuraminidase (NA) of seasonal influenza A viruses to cross-immunity against pH1N1 infection is unknown. Antibodies to the NA of different seasonal H1N1 influenza strains were test...
Kim, Hj Nader Pourmand
Published in
PLoS ONE
Correctly matching the HLA haplotypes of donor and recipient is essential to the success of allogenic hematopoietic stem cell transplantation. Current HLA typing methods rely on targeted testing of recognized antigens or sequences. Despite advances in Next Generation Sequencing, general high throughput transcriptome sequencing is currently underuti...
Kato-Maeda, M Ho, C Passarelli, B Banaei, N Grinsdale, J Flores, L Anderson, J Murray, M Rose, G Kawamura, Lm
...
Published in
PLoS ONE
Aguilar-Yáñez, Jm Portillo-Lara, R Mendoza-Ochoa, Gi García-Echauri, Sa López-Pacheco, F Bulnes-Abundis, D Salgado-Gallegos, J Lara-Mayorga, Im Webb-Vargas, Y León-Angel, Fo
...
Published in
PLoS ONE
Akhras, Ms Thiyagarajan, S Villablanca, Ac Davis, Rw Nyrén, P Nader Pourmand
Published in
PLoS ONE
The Molecular Inversion Probe (MIP) assay has been previously applied to a large-scale human SNP detection. Here we describe the PathogenMip Assay, a complete protocol for probe production and applied approaches to pathogen detection. We have demonstrated the utility of this assay with an initial set of 24 probes targeting the most clinically relev...
Akhras, Michael S Pettersson, Erik Diamond, Lisa Unemo, Magnus Okamoto, Jennifer Davis, Ronald W Pourmand, Nader
Published in
PloS one
Here we describe the novel Sequencing Bead Array (SBA), a complete assay for molecular diagnostics and typing applications. SBA is a digital suspension array using Next-Generation Sequencing (NGS), to replace conventional optical readout platforms. The technology allows for reducing the number of instruments required in a laboratory setting, where ...
Akhras, Ms Unemo, M Thiyagarajan, S Nyrén, P Davis, Rw Fire, Az Nader Pourmand
Published in
PLoS ONE
We combined components of a previous assay referred to as Molecular Inversion Probe (MIP) with a complete gap filling strategy, creating a versatile powerful one-primer multiplex amplification system. As a proof-of-concept, this novel method, which employs a Connector Inversion Probe (CIPer), was tested as a genetic tool for pathogen diagnosis, typ...
Nader Pourmand Diamond, L Garten, R Erickson, Jp Kumm, J Donis, Ro Davis, Rw
Published in
PLoS ONE
A highly discriminative and information-rich diagnostic assay for H5N1 avian influenza would meet immediate patient care needs and provide valuable information for public health interventions, e.g., tracking of new and more dangerous variants by geographic area as well as avian-to-human or human-to-human transmission. In the present study, we have ...
Toppozini, L Dies, H David Deamer Rheinstädter, Mc
Published in
PLoS ONE
A fundamental question of biology is how nucleic acids first assembled and then were incorporated into the earliest forms of cellular life 4 billion years ago. The polymerization of nucleotides is a condensation reaction in which phosphodiester bonds are formed. This reaction cannot occur in aqueous solutions, but guided polymerization in an anhydr...